This is a quote from the “Brine Shrimp Direct” web page. http://www.brineshrimpdirect.com/Hatching-Brine-Shrimp-Cysts-c169.html
“Hatching Environment Follow these guidelines for the best results:
25 parts per thousand (ppt) salt solution, or approximately 1 and 2/3 tablespoons of salt per quart (or liter) of water. This equates to around 1.018 specific gravity as measured with a hydrometer. Be sure to use marine salt or solar salt.
Proper pH is important in hatching brine shrimp. A starting pH of 8.0 or higher is recommended. In areas where the water pH is below 7, Epson salt or magnesium sulfate can be added at the rate of 1/2 teaspoon per quart of solution to buffer the hatching solution.
Optimum water temperature for a 24-hour complete hatch is 80-82°F or 26-28°C. Lowering the temperature would result in a longer hatching time. Do not exceed 30°C.
Illumination is necessary to trigger the hatching mechanism within the embryo during the first few hours of incubation. Maintaining a light source during the entire incubation period is recommended to obtain optimum hatch results and for temperature control.
Constant aeration is necessary to keep cysts in suspension and to provide sufficient oxygen levels for the cysts to hatch. A minimum of 3 parts per million dissolved oxygen during the incubation is recommended. Strong aeration should not damage or hurt the brine shrimp cysts or nauplii.
- Stocking Density:
1 gram per liter or quart or approximately 1/2 level teaspoon of cysts per quart is recommended. A higher stocking density will result in a lower hatch percentage.
- Hatching Cone:
Flat-bottom hatching vessels should be avoided. Cone or “V” bottomed containers are best to insure that the cysts remain in suspension during hatching. Be sure to thoroughly wash the hatching cone with a light chlorine solution, rinse, and allow to air-dry between uses. Avoid soap. Soap will leave a slight residue which will foam from aeration during hatching and leave cysts stranded above the water level.
- Incubation Period:
Generally, the optimum incubation time is 24 hours. Egg which has been properly stored for more than 2-3 months may require additional incubation time — up to 30-36 hours. Oftentimes, eggs will hatch in as few as 18 hours. If a smaller size nauplii (Instar I) is desired, a harvest time of 18 hours is recommended.”
This is the most complete set of instructions I have found. This is a reputable company which I have been using for brine shrimp for a while now. The instructions are geared more for the large fish breeders so you will have to scale it down if you are like me with a few tanks and small amounts of fish to raise. For convenience, (or laziness), I don’t always follow their directions exactly but they seem to have done some experimenting so I think they know what they are talking about.